The project entitled “Determining the impact of registered and non-registered fungicides and disinfestants on Uromyces transversalis, the causal agent of gladiolus rust, and their use as mitigation tools in combination with host plant resistance,” had eight objectives with the primary goal of developing strategies to locally eradicate gladiolus rust, caused by Uromyces transversalis. Fungicides individually and in programs were evaluated and recommendations were included for Mexican growers and two growers in California. Four experiments were conducted screening gladiolus cultivars with more tolerant cultivars identified. In addition to examining survivability of urediniospores in the field, survival of spores under controlled environmental conditions has been examined. Polyclonal antibodies were developed from germinating urediniospores and based on antigens identified through development of cDNA libraries.